CRYOPRESERVATION
Long-term Preservation Medium for Hepatic Cells (KRYO PRO™)
Versions
- Kryo Pro - I; our most efficient preservation medium for many cell types.
- Kryo Pro - II; a less expensive preservation medium with potential freezing success for many cell types.
"Kryo Pro" is our global name for cell cryopreservation media. Variations in cryo-protecting and functional cell outcomes are attributed to many factors including initial tissue qualities and native cell performances. Due to cellular diversity (phenotypic differences), cell responses to cryoprotectant media are unique. Cell background differences may include age, gender, DMI, diabetic, hypertensive, steatotic levels, and others; each with specific cryo support needs.
CRYO – THAW
Thawing Medium for Hepatic Cells (KRYO THAW™)
Versions
- Kryo Thaw - I; for human hepatocytes or difficult to thaw cell phenotypes.
- Kryo Thaw – II; for cells needing slight quality improvements, starting with the cyro-thaw process.
"Kryo Thaw" is our global name for cell cryopreservation thawing media. "Kryo Thaw" product goals are to improve cryopreserved post-thaw cell efficacies. More information is available in our Kryo Thaw brochure.
Kryo Thaw I and/or II is now available in "ready to use" centrifuge tubes (15 or 50 ml) or larger container formats.
For all orders and inquiries, please contact
info@scikoninovation.com or 919.354.1083
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General Cost Structure
| Cryopreservation | Unit Label | Cost |
| Kryo Pro I – 50ml | A | $ 185 |
| Kryo Pro I – 100ml | B | $ 345 |
| Kryo Pro II – 50ml | C | $ 125 |
| Kryo Pro II – 100ml | D | $ 195 |
| KRYO THAW™ | Unit Label | Cost |
| Kryo Thaw I– 12ml | A | $ 28 |
| Kryo Thaw – I – 40ml | A | $ 55 |
| Kryo Thaw I – 100ml | A | $ 105 |
| Kryo Thaw I – 250ml | A | $ 265 |
| Kryo Thaw II – 12ml | A | $ 24 |
| Kryo Thaw II– 40ml | A | $ 51 |
| Kryo Thaw II– 100ml | A | $ 98 |
| Kryo Thaw II– 250ml | A | $ 248 |
| Kryo Thaw I - 100ml | B | $ 130 |
| Kryo Thaw I - 250ml | B | $ 295 |
| Kryo Thaw II - 100ml | B | $ 121 |
| Kryo Thaw II - 250ml | B | $ 275 |
| Lot Number | Unit Label | Container | Cell | Sterile | Phenol Red Free | Ready to use | Shelf Life | Cold Stored | Info |
| Kryo Pro - I | A / B | bottles | cryo | yes | yes | yes | 6 months | 4 deg. C | |
| Kryo Pro - II | C / D | bottles | cryo | yes | yes | yes | 2 months | 4 deg. C | |
| Kryo Thaw I | A | varies | thaw | yes | yes | yes | 15 days (premixed) | 4 deg. C | |
| Kryo Thaw II | B | varies | thaw | yes | yes | yes | 6 months (2-parts) |
4 deg. C |
Cryopreservation Background: In many instances, freezing techniques are used to preserve living cells. Unfortunately, the cryopreservation and recovery (thaw) of some cells has proven to be quite troublesome. During tissue procurement and cell processing, cells are subjected to relatively harsh conditions, followed by freeze-thaw cycles that result in decreased cell survival rates. Techniques of cryopreservation may induce three modes of cell decay:
- Physical cell rupture caused by the effects of cellular volume changes and intercellular ice crystal formation.
- Cell necrosis which occurs rapidly and is characterized by cellular swelling, compromised cell membrane integrity, random DNA fragmentation, and release of cytokines.
- Apoptosis, which has been identified as a major cause of cryopreservation-induced cell death.
KRYO PRO™ and KRYO THAW™ are medium buffers used to help reduce cell decay responses. Subsequently, for post-thaw activities, plating efficiencies are influenced by cryo-components. These buffers are formulated to help meet the "off-the-shelf" cell demand; as cryopreserved cells are an ideal product choice.